Textile Engineering, Chemistry and Science
Textiles Complex 3328
Dr. Yang Zhang develops fluorescent & stimuli-responsive materials and single-molecule methods to color-map and engineer the nanoscopic biological and man-made world, especially nanofiber networks in the subcellular level including DNA, cytoskeleton and cell membranes. We are also interested in imaging biocompatible textile-nanofiber and understanding their nanoscopic structure-function relationships in biomedical and energy-related applications.
Detailed research directions please visit our group page https://sites.google.com/view/mapperncsu
PhD Chemistry University of Miami 2017
Postdoc Biomedical Engineering Northwestern University 2019
- Photoactivatable BODIPYs for Live-Cell PALM , MOLECULES (2023)
- Experimental Parameters-Based Monte-Carlo Simulation of Single-Molecule Localization Microscopy of Nuclear Pore Complex to Evaluate Clustering Algorithms , (2022)
- Minimizing Molecular Misidentification in Imaging Low-Abundance Protein Interactions Using Spectroscopic Single-Molecule Localization Microscopy , Analytical Chemistry (2022)
- Investigating Single-Molecule Fluorescence Spectral Heterogeneity of Rhodamines Using High-Throughput Single-Molecule Spectroscopy , The Journal of Physical Chemistry Letters (2021)
- Accelerating multicolor spectroscopic single-molecule localization microscopy using deep learning , Biomedical Optics Express (2020)
- Compact, “Clickable” Quantum Dots Photoligated with Multifunctional Zwitterionic Polymers for Immunofluorescence and In Vivo Imaging , Bioconjugate Chemistry (2020)
- Live-Cell Imaging at the Nanoscale with Bioconjugatable and Photoactivatable Fluorophores , Bioconjugate Chemistry (2020)
- Photoactivatable fluorophores for single-molecule localization microscopy of live cells , Methods and Applications in Fluorescence (2020)
- Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction , Langmuir (2020)
- High-Throughput Single-Molecule Spectroscopy Resolves the Conformational Isomers of BODIPY Chromophores , The Journal of Physical Chemistry Letters (2019)
The goal of this project is to generate a library of cell-permeable and bioconjugatable synthetic dyes with photoactivatable fluorescence and resolved single-molecule emission spectra. Their design is based on a photochemical mechanism to activate borondipyrromethene (BODIPY) fluorescence with green light developed in our prior funding cycle. Their realization demands the synthesis, structural characterization, ensemble/single-molecule spectroscopic analysis and imaging of four series of photoactivatable BODIPYs, differing in the ring-fusion pattern of their polycyclic chromophoric scaffold and/or nature of their substituents, in two parallel project components. One is aimed at resolving the spectral position and/or band shape of the photoactivated fluorescence to differentiate the single-molecule emission spectra sufficiently for structural discrimination with statistical confidence. The other is aimed at introducing hydrophilic groups and targeting ligands on the chromophoric platform to label selectively and track simultaneously intracellular proteins. The overall outcome will be the engineering of a unique combination of structural, photochemical and photophysical properties into innovative molecular constructs to enable the unprecedented high-throughput multiplexed single-molecule tracking of proteins in live cells.
This project aims to develop a palette of bright switchable fluorescent probes for simultaneous multiplexed super-resolution imaging at the molecular level. We will increase the photon emission rate of the newly-designed switchable dyes and encode distinct spectral features of the switchable dyes in the two specific aims to collectively provide high resolution and multiplexity. This project will allow biomedical researchers, for the first time, to achieve simultaneous multi-color imaging of 6 molecular labels at 10-nm spatial resolution within 10 minutes. System performance will be investigated by imaging nanoscale engineered samples, and multiple molecular targets with distinct morphologies in cells.
The objective of the proposal is to develop a palette of synthetic photoactivatable fluorophores (PAFs) for live-cell multiplexed spectroscopic single-molecule tracking of multiple (ï‚³4) biomolecules simultaneously with 10-nm spatial precision and 10-ms temporal resolution. In particular, the specific aims of this proposal are (1) to synthesize visible-light activatable and bioconjugatable PAFs for spt-PALM (referred to single particle tracking-Phoactivated localization microscopy) with minimal photocytotoxicity and (2) to synthesize PAFs with resolved emission peaks in the red spectral region for simultaneously multiplexed spt-PALM. The photogenerated species of the photoactivatable probes with distinct yet partially-resolved emission spectra will be concurrently localized and distinguished using spectroscopic single-molecule localization microscopy (sSMLM) to achieve multiplexed spt-PALM, thus enabling simultaneous multi-color super-resolution imaging in live cells. The grantee shall perform the single-molecule spectroscopic characterization of the resulting switchable probes after available and super-resolution imaging experiments in live cells. The specific goals for the collaborative research activity at NCSU are (1) the high-throughput measurement of single-molecule fluorescence spectral heterogeneity of the photogenerated species to guide the synthesis of a quartet of photoactivatable BODIPY-oxazine dyads that can be unambiguously distinguished; (2) the acquisition of single-color spt-PALM in live cells of specific subcellular organelles labeled with the developed switchable probes and activate by green light; (3) the development of imaging method to simultaneously track 4 photoactivated BODIPY chromophores and imaging analytics; (4) perform parallel multiplexed sSMLM-based spt-PALM of multiple subcellular organelles in live cells.